Erster funktionierender Myostatisblocker

Erstmal hallo, bin neu hier...

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Biol Chem. 2002 Oct 25;277(43):40735-41. Epub 2002 Aug 22.

The myostatin propeptide and the follistatin-related gene are inhibitory binding proteins of myostatin in normal serum.

Hill JJ, Davies MV, Pearson AA, Wang JH, Hewick RM, Wolfman NM, Qiu Y.

Department of Protein Chemistry and Proteomics, Wyeth Research, 87 Cambridge Park Drive, Cambridge, MA 02140, USA. jhill@wyeth.com

Myostatin, also known as growth and differentiation factor 8, is a member of the transforming growth factor beta superfamily that negatively regulates skeletal muscle mass (1). Recent experiments have shown that myostatin activity is detected in serum by a reporter gene assay only after activation by acid, suggesting that native myostatin circulates as a latent complex (2). We have used a monoclonal myostatin antibody, JA16, to isolate the native myostatin complex from normal mouse and human serum. Analysis by mass spectrometry and Western blot shows that circulating myostatin is bound to at least two major proteins, the myostatin propeptide and the follistatin-related gene (FLRG). The myostatin propeptide is known to bind and inhibit myostatin in vitro (3). Here we show that this interaction is relevant in vivo, with a majority (>70%) of myostatin in serum bound to its propeptide. Studies with recombinant V5-His-tagged FLRG protein confirm a direct interaction between mature myostatin and FLRG. Functional studies show that FLRG inhibits myostatin activity in a reporter gene assay. These experiments suggest that the myostatin propeptide and FLRG are major negative regulators of myostatin in vivo.


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Human Growth Factors & Cytokines

Recombinant Human Myostatin-Propeptide



Description :

Mature Myostatin is obtained by proteolytic processing of a biologically-inactive precursor protein, which contains an N-terminal propeptide of 243 amino acid residues. Myostatin Propeptide exhibits high binding affinity for myostatin and has been shown to be a potent inhibitor of Myostatin. Over-expression of myostatin propeptide in mice resulted in large increases (up to 200%) in skeletal muscle mass, similar to those observed in Myostatin knockout mice. Recombinant Human Myostatin Propeptide is a 27.8 kDa protein consisting of 244 amino acid residues.

Catalog #:

120-12

Source :

E.coli

Stability :

The lyophilized protein is stable for a few weeks at room temperature, but best stored at -200C. Reconstituted Myostatin Propeptide should be stored in working aliquots at -200C.

Purity :

Greater than 98% by SDS-PAGE.

Endotoxin Level :

Endotoxin level is less than 0.1 ng per µg (1EU/µg).

Biological Activity :

Testing in progress.

AA Sequence :

MNENSEQKEN VEKEGLCNAC TWRQNTKSSR IEAIKIQILS KLRLETAPNI SKDVIRQLLP KAPPLRELID QYDVQRDDSS DGSLEDDDYH ATTETIITMP TESDFLMQVD GKPKCCFFKF SSKIQYNKVV KAQLWIYLRP VETPTTVFVQ ILRLIKPMKD GTRYTGIRSL KLDMNPGTGI WQSIDVKTVL QNWLKQPESN LGIEIKALDE NGHDLAVTFP GPGEDGLNPF LEVKVTDTPK RSRR



5µg$75 -------25µg$185--------1mg$4800

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the antibody polyclonal:

Product Name
GDF8 antibody

Catalog Number
GTX30381

Product Description:
Anti - GDF8 polyclonal

Manufacturer & Mfg Part#:
Novus NB 100-281


Cross Reactivity:
This product recognizes human and mouse GDF8. Other species have not been tested.

Storage Instruction:
Store at 2-8 °Celcius.

Concentration:
1 mg/ml

Clonality:
POLYCLONAL

Tested Applications:
Immunohistochemistry, Western blot

Form:
Liquid

Specificity:
This antibody is specific for human GDF8.

Concentration Unit:
mg/ml

Immunogen:
A synthetic peptide, which represented a portion of human Growth Differentiation Factor 8 encoded within exon 3 (LocusLink ID 2660).

$ 2750 a mg.





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Sollte das bei Menschen angewendet werden wirds schon pervers. Hier Bilder von betroffenen Tieren:

http://www.owlnet.rice.edu/~bios201/images/myostatin1.jpg

http://fig.cox.miami.edu/~cmallery/150/neuro/belgian.blue.jpg

http://www.ultimate-exercise.com/images/mouse_b.jpg

Gruß,
Kawa